Detection of nitric oxide by electron paramagnetic resonance spectroscopy

Electron Paramagnetic Resonance Spectroscopy

Demystifying EPR: A Rookie Guide to the Application of Electron Paramagnetic Resonance Spectroscopy on Biomolecules

Electron Paramagnetic Resonance (EPR) spectroscopy, also known as Electron Spin Resonance(ESR) especially among physicists, is a strong and versatile spectroscopic method forinvestigation of paramagnetic systems, i.e. systems like free radicals and most transition metalions, which have unpaired electrons. The sensitivity and selectivity of EPR are notable andintriguing as compared to other spec...

Hyperthermia stimulates nitric oxide formation: electron paramagnetic resonance detection of .NO-heme in blood.

Previous experiments from our laboratory have demonstrated that severe hyperthermia results in a selective loss of splanchnic vasoconstriction. Using electron paramagnetic resonance spectroscopy to scan whole blood samples collected in vivo from the portal vein and femoral artery of conscious unrestrained rats, we observed an increase in the concentration of spectroscopy-detectable species in p...

Paramagnetic resonance study of Nitric Oxide hemoglobin.

The electron paramagnetic resonance absorption of nitric oxide hemoglobin was observed as a possible model system for oxyhemoglobin. The electron paramagnetic resonance spectrum showed three g factors indicating a rhombic symmetry around the paramagnetic center. By use of 15N0, the unpaired electron was found very little associated with NO nitrogen, in contrast to what has been customarily assu...

New methods to evaluate endothelial function: Evaluation of endothelial function by hemoglobin-nitric oxide complex using electron paramagnetic resonance spectroscopy.

This minireview describes the practical use of assay systems to detect nitric oxide (NO) by electron paramagnetic resonance (EPR) spectroscopy for evaluation of endothelial functions. The iron(II)-dithiocarbamate complexes, such as iron(II)-(N-methyl-D-glucamine dithiocarbamate), are commonly used in EPR detection of NO both in vivo and in vitro. However, due to their redox activity, these comp...